Antisense Technology

Configuration of the 5'-Methyl Group Modulates the Biophysical and Biological Properties of Locked Nucleic Acid (LNA) Oligonucleotides

Seth PP, Allerson CR, Siwkowski A, Vasquez G, Berdeja A, Migawa MT, Gaus H, Prakash TP, Bhat B, Swayze EE

J Med Chem. 2010 Nov 8;53(23):8309–8318

Polypurine-Repeat-Containing RNAs: a Novel Class of Long Non-Coding RNA in Mammalian Cells

Zheng R, Shen Z, Tripathi V, Xuan Z, Freier SM, Bennett CF, Prasanth SG, Prasanth KV

Cell Sci. 2010 Nov 1;123(Pt 21):3734-44

The Nuclear-Retained Noncoding RNA MALAT1 Regulates Alternative Splicing by Modulating SR Splicing Factor Phosphorylation

Tripathi V, Ellis JD, Shen Z, Song DY, Pan Q, Watt AT, Freier SM, Bennett CF, Sharma A, Bubulya PA, Blencowe BJ, Prasanth SG, Prasanth KV

Mol Cell. 2010 Sep 24;39(6):833-4

RNA Targeting Therapeutics: Molecular Mechanisms of Antisense Oligonucleotides as a Therapeutic Platform

Bennett CF, Swayze EE

Annu Rev Pharmacol Toxicol. 2010;50:259-93

Off-target and a Portion of Target-specific siRNA Mediated mRNA Degradation is Ago2 'Slicer' Independent and Can be Mediated by Ago1

Vickers TA, Lima WF, Wu H, Nichols JG, Linsley PS, Crooke ST

Nucleic Acids Res. 2009 Nov;37(20):6927-41

Binding and Cleavage Specificities of Human Argonaute2

Lima WF, Wu H, Nichols JG, Sun H, Murray HM, Crooke ST

J Biol Chem. 2009 Sep 18;284(38):26017-28.

Potent Inhibition of microRNA in vivo without Degradation.

Davis S, Propp S, Freier S, Jones L, Serra M, Kinberger G, Bhat B, Swayze E, Bennett F, Esau C.

Nucleic Acids Res. 2009 Jan;37(1):70-7.

Human Dicer Binds Short Single-strand and Double-strand RNA with High Affinity and Interacts with Different Regions of the Nucleic Acids.

Lima WF, Murray H, Nichols JG, Wu H, Sun H, Prakash TP, Berdeja AR, Gaus HJ, Crooke ST.

J Biol Chem. 2008 November [Epub ahead of print].

3' End Processing of a Long Nuclear-retained Noncoding RNA Yields a tRNA-like Cytoplasmic RNA.

Wilusz JE, Freier SM, Spector DL.

Cell. 2008 November;135(5):919-932.

MicroRNA 21 Promotes Glioma Invasion by Targeting Matrix Metalloproteinase Regulators.

Gabriely G, Wurdinger T, Kesari S, Esau CC, Burchard J, Linsley PS, Krichevsky AM.

Mol Cell Biol. 2008 Sep;28(17):5369-80.

Antisense Transcripts Are Targets for Activating Small RNAs

Schwartz JC, Younger ST, Nguyen N, Hardy DB, Monia BP, Corey DR, Janowski BA.

Nature Structural & Molecular Biology . 2008 August;15(8):842-848.

Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice.

Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR.

AJHG. 2008 April 11;82(4):834-848.

Inhibition of microRNA with antisense oligonucleotides.

Esau CC.

PMID. 2008 Jan;44(1):55-60.

Reduced Levels of Ago2 Expression Result in Increased siRNA Competition in Mammalian Cells

Vickers TA, Lima WF, Nichols JG, Crooke ST

Nucleic Acids Res. 2007;35(19):6598-610

MicroRNA-143 Regulates Adipocyte differentiation.

Esau C, Kang X, Peralta E, Hanson E, Marcusson EG, Ravichandran LV, Sun Y, Koo S, Perera RJ, Jain R, Dean NM, Freier SM, Bennett CF, Lollo B, Griffey R.

J Biol Chem. 2004 Dec 10;279(50):52361-5.

Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents. A comparative analysis.

Vickers, T.A., Koo, S., Bennett, C.F., Crooke, S.T., Dean, N.M., Baker, B.F.

J Biol. Chem. 278(9):7108-18, 2003.

Molecular mechanisms of action of antisense drugs

Crooke, S.T.

Biochim Biophys Aca.1489 (1): 31-43, 1999.

Induction of endogenous Bcl-xS through the control of Bcl-x pre-mRNA splicing by antisense oligonucleotides.

Taylor, J. K., Zhang, Q. Q., Wyatt, R. and Dean, N. M

Nature Biotechnology.17(11):1097-1100, 1999.

Properties of cloned and expressed human RNase H.

Wu, H., Lima, W.F. and Crooke, S.T.

J. Biol. Chem. 274(40)28270-28278, 1999.